Repressor element-1 silencing transcription factor (REST) is a transcriptional repressor of neuron-specific genes that binds to a conserved DNA element, the neuron restrictive silencer element (NRSE/RE1). Interestingly, increased REST activity is found in several neurological diseases like Huntington's disease and cerebral ischemia.

RNA-containing NRSE sequence forms double-stranded Neuronal Lineage Induction by the NRSE dsRNA RNA (dsRNA) in lengths of about 20 bp, and that the To determine the function of the NRSE dsRNA, we ex- NRSE dsRNA activates expression of NRSE / RE1 -con- pressed them in HCN-A94 cells.

In non-neuronal cells, neuron-restrictive silencer factor (NSRF) actively represses gene transcription via a sequence-specific DNA motif known as the neuron-restrictive silencer element (NRSE). This DNA motif has been identified in many genes that are specific markers for cells of neuronal and neuroendocrine lineage. We found that the mouse Na v 1.8 gene contains two putative conserved NRSE sites, a forward-oriented sequence within the 5′-untranslated region (NRSE-1) and a reverse-oriented sequence within intron 10 (NRSE-2) (Fig. 1 B, C). To better restrict transgene expression to the nervous system in zebrafish larvae, we have used DNA sequences derived from the neuron-restrictive silencing element (NRSE). We find that one such sequence, REx2, when used in conjunction with several basal promoters, robustly suppresses transgene expression in non-neuronal tissues.

In non-neuronal cells, neuron-restrictive silencer factor (NSRF) actively represses gene transcription via a sequence-specific DNA motif known as the neuron-restrictive silencer element (NRSE). This DNA motif has been identified in many genes that are specific markers for cells of neuronal and neuroendocrine lineage. Neuron-restrictive silencer factor has been shown to bind to the NRSE sequence within the MOR gene and play important roles in modulating the expression of the MOR gene. 8,18,19,47 Next, we investigated the expression of NRSF in the ipsilateral spinal dorsal horn or L2-4 DRG after sarcoma cell inoculation at both transcriptional and translational levels. NRSE-like sequences are present in the human CYP11B2 (hCYP11B2) and CYP11B1 (hCYP11B1) genes. A homology search for NRSE-like sequences identified NRSE-like sequences within the genomic sequence corresponding to intron 8 and exon 9 of the human genes but not in those of other species ( Supplemental Table 2 ). 2006-07-09 · Using comparative sequence analysis, here we report the identification of 895 sites (NRSE) as the putative targets of REST.

1, upper panel). As shown in the inset of Fig. 1 , the NRSE-like sequence identified within the intron of the HCN4 gene is highly conserved among several species and is homologous to the consensus NRSE, suggesting that it might mediate an important regulatory function in the HCN4 transcription.

A, the NRSE sequence of the mouse MOR is very similar to a consensus NRSE, and it is highly conserved among at least three species, mouse, rat, and human. The NRSE is located from –12 to +9 on the MOR promoter and contains a translation start site (ATG).

The NRSF/REST sequence-tag distribution centers directly over the only canonical NRSE motif in a 4-kb region, which is located in the open reading frame of the NEUROD1 gene. This site was called a ChIPSeq peak by the locator algorithm [open source available at ( 14 )]. The consensus sequence of the Xenopus NRSE varies slightly from that of human.

while a third type features half-site RE1/NRSE with only one-half of the canonical RE1/NRSE.7 Of note, variations of the RE1/NRSE sequence are associated with modulation of REST/NRSF affinity to the respective DNA site, allowing some RE1/NRSE sites to be occupied by REST/NRSF, while others may remain unbound in the same cell.8 RE1/NRSE sites

We find that one such sequence, REx2, when used in conjunction with several basal promoters, robustly suppresses transgene expression in non-neuronal tissues. The sequence analysis of HCN4 gene revealed the presence of a conserved NRSE motif, which is known to bind the transcriptional factor neuron-restrictive silencing factor (NRSF).

They noted that the SCG10 regulatory region contains both activation and repression (i.e., silencer) domains and that similar NRSE-like sequence elements have been identified in other neuron-specific genes. NRSE sequences were effective in restricting expression of bipartite Gal4-based ‘driver’ transgenes within the context of an enhancer trap and when associated with a defined promoter and enhancer. However, NRSE sequences did not serve to restrict expression of an upstream activating sequence (UAS)-based reporter/effector 2001-11-01 · Sequence analysis of this promoter region revealed the presence of a neuron-restrictive silencer element (NRSE) known to bind repressor zinc finger protein REST. This factor is not expressed in insulin-secreting and neuron-like cells.
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This DNA motif has been identified in many genes that are specific markers for cells of neuronal and neuroendocrine lineage.
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The consensus sequence of the Xenopus NRSE varies slightly from that of human. For example, whereas nucleotide A is predominant at position 7 in the human NRSE consensus, both A and G are in high occupancy at this position in Xenopus (chi-square test, p < 1.0E-6) (Figure 1 A).

Of these, 892, 944, and 291 are found in the human, mouse, and Fugu genomes, respectively. The frequency at which each RE1 variation occurred in the human genome was compared to the frequency in the mouse and Fugu genomes (see Table 1).

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Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence. SnapGene File: Plasmid sequence and SnapGene enhanced annotations. Use with SnapGene software or the free Viewer to visualize additional data and align other sequences.

When MOR and NRSF genes were coexpressed, the specific ligand-binding activity of MOR was increased in neuroblastoma NMB cells, but decreased in PC12 cells result from its localization. 2006-11-27 · From the sequence data analysis, we initially found the conserved Sp family binding site adjacent to NRSE in mouse, rat and human MOR gene that could regulate MOR gene expression. When treated with mithramycin A, a G/C box specific binding inhibitor, the mRNA level of MOR gene was increased in NRSF containing NS20Y cells but not in NRSF negative PC12 cells ( Figure 1 ). NRSE is a regulatory sequence that is present in several neuronal genes and that was, up to now, thought to silence neuronal gene transcription in nonneuronal cells (6, 7, 9–14). We have analyzed the function of NRSE as well as the importance of its position in the promoter of the gene encoding the mouse nAChR β2-subunit and in synthetic promoters by combining transgenic mice and in vitro Based on a previous report indicating the presence of a NRSE in the Na v 1.8 gene (Otto et al., 2007), we analyzed its location and sequence. We found that the mouse Na v 1.8 gene contains two putative conserved NRSE sites, a forward-oriented sequence within the 5′-untranslated region (NRSE-1) and a reverse-oriented sequence within intron 10 (NRSE-2) ( Fig. 1 B , C ).