Label-free concentration of viable neurons, hESCs and cancer cells by means of acoustophoresis† Marina C. Zalis , a Juan F. Reyes , b Per Augustsson , c Staffan Holmqvist , d Laurent Roybon , d Thomas Laurell c and Tomas Deierborg * a

Microfluidic, Label-Free Enrichment of Prostate Cancer Cells in Blood Based on Acoustophoresis Forskningsoutput : Tidskriftsbidrag › Artikel i vetenskaplig tidskrift Översikt

Projekt: Evaluation and optimization of active surveillance for men living with clinically insignificant prostate cancer. Researchers from MIT, Pennsylvania State University, and Carnegie Mellon University have devised a new way to separate cells by exposing them to sound waves They have presented an acoustophoresis device with trifurcation inlet and outlet to separate three different prostate cancer cell lines (DU145, PC3, and LNCaP) from no-cancer control subjects’ blood sample spiked with the mentioned cancer cells . Second part of the thesis focuses on acoustophoresis. In (paper 5), a multifunctional acoustic microdevice was developed for isolation of cancer cells from red blood cells with a separation efficiency of 92.4% and trapping efficiency of 93%. In (paper 6), microbubbles activated acoustic cell sorter was developed for affinity based cell separation. At a flow rate of 100 μL/min, breast cancer cells (MCF7) spiked into red blood cell-lysed human blood were separated with an efficiency of 91.8 ± 1.0% with a contamination of 0.6 ± 0.1% from white blood cells with a 23.8 ± 1.3-fold concentration of cancer cells. Resistive pulse sensing (RPS) is a key label-free technology to measure particles and single-cell size distribution.

Our studies indicate that microchannel acoustophoresis can be tuned for effective cell handling without having any effect of cell viability, proliferation, respiration or inflammatory status. Materials and Methods Ethics Statement Label-free concentration of viable neurons, hESCs and cancer cells by means of acoustophoresis† Marina C. Zalis , a Juan F. Reyes , b Per Augustsson , c Staffan Holmqvist , d Laurent Roybon , d Thomas Laurell c and Tomas Deierborg * a Using this microchannel configuration, several publications have shown cancer cell lines (breast and prostate) being efficiently separated from WBCs from RBC-lysed blood using acoustophoresis with BASWs [3, 20].This label-free separation occurs because larger-sized cancer cells focus faster to the center pressure node than WBCs in the separation channel; thus, if flow rates are optimized the WBCs will flow through the side outlet channel and cancer cells will be enriched in the collected Figure 4: Percentage of viable cells after one passage through the acoustophoresis channel. CONCLUSION We believe that this work contributes principal proof of concept showing that acoustophoresis has potential for use in clinical diagnostics of CTCs in cancer patients. Moreover, the modular selection criteria allows for either high clearance Microfluidic, label-free enrichment of prostate cancer cells in blood based on acoustophoresis. Circulating tumor cells (CTC) are shed in peripheral blood at advanced metastatic stages of solid cancers.

KEYWORDS: Micro-PIV, Ultrasound Resonances, Acoustophoresis, Cell Handling INTRODUCTION Microchannel acoustophoresis is a rapidly expanding research ﬁeld allowing gentle and efﬁcient manipulation of cells and other biological particles [1, 2].

Second part of the thesis focuses on acoustophoresis. In (paper 5), a multifunctional acoustic microdevice was developed for isolation of cancer cells from red blood cells with a separation efficiency of 92.4% and trapping efficiency of 93%. In (paper 6), microbubbles activated acoustic cell sorter was developed for affinity based cell separation.

"Microfluidic, Label-Free Enrichment of Prostate Cancer Cells in Blood Based on Acoustophoresis". Microfluidic, Label-Free Enrichment of Prostate Cancer Cells in Blood Based on Acoustophoresis Forskningsoutput : Tidskriftsbidrag › Artikel i vetenskaplig tidskrift Översikt The acoustophoresis configuration in Figure 3 C is especially well suited for removing dissolved substances, that is, medium exchange of the suspended cells. The cells enter via the side inlets and a clean buffer is delivered in the center flow path of the acoustophoresis channel.

Similar rates of cancer-cell recovery, cancer-cell purity, and fold-enrichment were seen with both prostate cancer (DU145, PC3) and breast cancer (MCF7) cell line cells. We identified eosinophil granulocytes as the predominant white blood cell (WBC) contaminant (85%) in the enriched cancer-cell fraction.

cell transplantation has developed as a standard treatment for many cancers besides development of several novel therapies for castrate-resistant prostate cancer, ultrasonic wave based microfluidic techniques (acoustophoresis) to enable. Umeå universitet, får 820 000 kronor. Projekt: Apheresis-acoustophoresis to improve cancer diagnosis in prostate cancer (APACA-PC). I have a broad skill set and I have so far used it mainly in cancer related applications.

Innocenti, Nicolas Jean-Amédée Multimodal Imaging and Acoustophoresis. Kuang, Qie. Structural Microchannel acoustophoresis has been evaluated for isolation of prostate and breast cancer cells from blood. Cancer cells are discriminated primarily based on their higher acoustophoretic mobility as compared to the white blood cells (WBCs). Influence of Acoustophoresis on Viability or Secretory Function of Prostate Cancer Cells. Acoustophoresis-based microfluidic devices are currently being explored as a mean to enrich and discriminate viable from non-viable tumor cells in peripheral blood .
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S. and Laurell T., Free flow acoustophoresis (FFA) – A imaging for breast cancer detection Malignancy Grading of Prostate Cancer Integration of acoustophoresis of whole blood with PSA diagnostics on protein.

The CTCs preserve primary tumor heterogeneity and mimic tumor properties, and may be considered as clinical biomarker Acoustophoresis Enables the Separation of Differently Sized Subsets of Cultured MSCs Cultured MSCs are known to be heterogeneous in cell size and other cellular properties. We found that MSC size heterogeneity increased over time in culture, that is, the mean MSC diameter in passage 3 was 14.2 ± 0.6 μm ( n = 3) and increased to 15.9 ± 0.9 μm in passage 4 ( n = 14, Fig. 1C ).
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Lung cancer is a leading type of cancer — and a leading killer — in the United States every year. The good news is many cases of lung cancer are believed to be preventable, as an estimated 90% of lung cancer cases are caused by active smoki

Chemical and Biological Microsystems Society. s. 432-434. (22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018).

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Whether colon cancer runs in your family or you’re interested in learning about health conditions as part of an effort to improve your well-being, it’s important to understand this type of cancer. According to the American Cancer Society, a

External funding 33.6 MSEK PI: Prof.